SPARC antibody

Catalog No. ABIN596878

This Goat Polyclonal antibody specifically detects SPARC in WB, ELISA and FACS. It exhibits reactivity toward Mouse.

Quick Overview for SPARC antibody (ABIN596878)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SPARC Antibody

Specificity: Recognizes mouse Osteonectin. Species cross-reactivity: less than 1 % cross-reactivity with recombinant human SPARC.

Purification: Immunoaffinity purified

Immunogen: Recombinant mouse Osteonectin.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (3 - 10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Flow Cytometry and Suitable for use in Western blot: 0.1-0.2 μg/mL. Flow Cytometry: 3-10 μg/mL per 10^6 cells. ELISA: 0.5-1 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C. Further dilutions can be made in buffer.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: Osteonectin / SPARC

Background: Name/Gene ID: SPARC

Synonyms: SPARC, Basement-membrane protein 40, BM-40, Cysteine-rich protein, ON, Osteonectin

Gene ID: 6678

UniProt: P09486

Pathways: Autophagy